Journal: International journal of oncology
Article Title: Macrophage-secreted IL-8 induces epithelial-mesenchymal transition in hepatocellular carcinoma cells by activating the JAK2/STAT3/Snail pathway.
doi: 10.3892/ijo.2014.2761
Figure Lengend Snippet: Figure 7. Role of IL-8 signaling in EMT. (A) The expression of Jak2, p-Jak2, Stat3, p-Stat3 and Snail were assessed in Hep-G2 exposure to different concentrations of IL-8. (B-D) Semi-quantitative analysis demonstrated that Jak2/Stat3/Snail pathway could be activated by IL-8. (#p>0.05; *p<0.05). (E) Immunofluorescent analysis of E-Cadherin, N-Cadherin and Snail in Hep-G2 vs. Hep-G2 that was incubated with IL-8. The green signal represents the staining of the corresponding protein and the blue signal represents the DAPI-stained nuclei. The morphological change of Hep-G2 after incubated with IL-8 was also observed. (F and G) Functional analysis is illustrated after IL-8 had been added to the media for 24 h. A transwell assay showed that the number of invading Hep-G2 cells were higher that in control groups (original magnification, x100).
Article Snippet: Rabbit anti-human JAK2/p-JAK2 monoclonal antibody (1:1,000; 3230; Cell Signaling Technology, Danvers, MA, uSA/1:1,000; 4406; Cell Signaling Technology) and rabbit anti-human STAT3/p-STAT3 monoclonal antibody (1:1,000; 4904; Cell Signaling Technology/1:2,000; 9145; Cell Signaling Technology) were selected to detect the JAK2/STAT3 pathway.
Techniques: Expressing, Incubation, Staining, Functional Assay, Transwell Assay, Control